• List of Contributors
• Preface
• Introduction to Biology
• Basic Concepts in Engineering Biology
• Foundational Technologies
• Minimal Cells and Synthetic Life
• Parts, Devices and Systems
• Modelling Synthetic Biology Systems
• Applications of Designed Biological Systems
• iGEM
• The Societal Impact of Synthetic Biology
• Appendices: Proforma of Common Laboratory Techniques
• Glossary
• Index--.
• (source: Nielsen Book Data)

Synthetic Biology - A Primer (Revised Edition) presents an updated overview of the field of synthetic biology and the foundational concepts on which it is built. This revised edition includes new literature references, working and updated URL links, plus some new figures and text where progress in the field has been made.The book introduces readers to fundamental concepts in molecular biology and engineering and then explores the two major themes for synthetic biology, namely 'bottom-up' and 'top-down' engineering approaches. 'Top-down' engineering uses a conceptual framework of systematic design and engineering principles focused around the Design-Build-Test cycle and mathematical modelling. The 'bottom-up' approach involves the design and building of synthetic protocells using basic chemical and biochemical building blocks from scratch exploring the fundamental basis of living systems.Examples of cutting-edge applications designed using synthetic biology principles are presented, including: the production of novel, microbial synthesis of pharmaceuticals and fine chemicals the design and implementation of biosensors to detect infections and environmental waste.The book also describes the Internationally Genetically Engineered Machine (iGEM) competition, which brings together students and young researchers from around the world to carry out summer projects in synthetic biology. Finally, the primer includes a chapter on the ethical, legal and societal issues surrounding synthetic biology, illustrating the integration of social sciences into synthetic biology research.Final year undergraduates, postgraduates and established researchers interested in learning about the interdisciplinary field of synthetic biology will benefit from this up-to-date primer on synthetic biology.
(source: Nielsen Book Data)

Today's synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts. Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges. Learn the fundamentals of biodesign and DNA engineering Explore important ethical issues raised by examples of synthetic biology Investigate the BioBuilder labs that probe the design-build-test cycle Test synthetic living systems designed and built by engineers Measure several variants of an enzyme-generating genetic circuit Model "bacterial photography" that changes a strain's light sensitivity Build living systems to produce purple or green pigment Optimize baker's yeast to produce ?-carotene.
(source: Nielsen Book Data)

• SECTION 1. Getting Oriented
• Chapter 1: General lab organization and procedures
• Chapter 2: Laboratory setup and equipment
• Chapter 3: Getting started and staying organized
• SECTION 2. Plotting a Course
• Chapter 4: How to set up an experiment
• Chapter 5: Laboratory notebooks
• Chapter 6: Presenting yourself and your data
• SECTION 3. Navigating
• Chapter 7: Making reagents and buffers
• Chapter 8: Storage and disposal
• Chapter 9: Working without contamination
• Chapter 10: Eukaryotic cell culture
• Chapter 11: Bacteria
• Chapter 12: DNA, RNA, and protein
• Chapter 13: Radioactivity
• Chapter 14: Centrifugation
• Chapter 15: Electrophoresis
• Chapter 16: Microscopy.
• (source: Nielsen Book Data)

At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures.
(source: Nielsen Book Data)